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KMID : 0356420010190010035
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Journal of Korean Andrology
2001 Volume.19 No. 1 p.35 ~ p.39
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Collagen Changes of the Corpus Caernosum in Streptozotocin-Induced Diabetic Rats and the Effects of Insulin and Aminoguanidine
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Kim Kun-Suk
Hong Jun-Hyuk
Ahn Tai-Young
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Abstract
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Purpose: Diabetes changes the properties of collagen in our body tissue, increasing cross-linked collagen via nonenzymatic glycosylation and accumulation of advanced glycosylation end products. Glucose-derived cross-linked collagen formation may contribute to the development of chronic diabetic complications such as decreasing the compliance of tissue. Aminoguanidine, a hydrazine compound, prevent formation of glucose-derived collagen cross-linking. We studied the change of the properties of collagen in the corpus cavernosum by diabetes and the effects of insulin and aminoguanidine.
Materials and Methods: Diabetes was induced in male Sprague-Dawley rat and maintained for 12 weeks. Rats were divideed into four groups: 1) control, 2) diabetics, 3) diabetics treated with insulin, 4) diabetics treated with aminoguanidine. Concentration of collagen in cavernosal tissue was measured by hydroxyproline content and expressed as hydroxyproline (㎍)/ dried defatted tissue (㎎). The characteristic autofluorescence of glycosylated connective tissues of penile tissue was used to quantitate advanced glycosylation end products.
Results: The content of collagen in cavernosal tissue was not significantly different among experimental groups. Cross-linked collagen expressed as fluorescence (U/㎍ hydroxyproline) was increased in diabetic group (61.6±5.1) compared to control group (24.1±3.2) and it was decreased in both insulin-treated group (36.9±7.4) and aminoguanidine-treated group (37.9±5.8) compared to diabetic group.
Conclusion: Our studies revealed that the properties of collagen in corpus cavernosum was changed by diabetes and that aminoguanidine as well as insulin prevented the diabetes-induced crossed linked collagen formation.
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KEYWORD
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Collagen, Diabetes mellitus, Aminoguanidine, Corpus cavernosum,
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